UTILIZATION OF RED DRAGON FRUIT PEEL NATURAL DYE IN SPERM EXAMINATION IN CLINICAL LABORATORY

Abstract

Examination of semen to evaluate fertility in a man includes examination to determine volume, pH, color, viscosity, liquefaction time, number of round cells, total sperm count, concentration, motility and normal sperm morphology. Sperm morphology is evaluated based on the presence of head and neck structures. WHO recommends two staining methods to evaluate sperm deformities, namely Papanicolaou and Diff-Quik staining. However, the use of synthetic dyes produces waste that can be detrimental to the environment and water sources. This study aims to identify and compare the quality of spermatozoa morphology staining results using natural ingredients from red dragon skin extract and Papanicolaou. Dragon fruit peel extract is made with variations in the solvent composition of ethanol, acetic acid and alum mordant. The results of the study showed that staining using red dragon skin extract gave very good results on sperm head and tail morphology, but less well on spermatozoa neck morphology. From the results of the non-parametric Friedman test, it was found that the Asymp.sig.(2-tailed) value was <0.05, which stated that there was a difference in the results of staining using Papanicolou and dragon fruit peel. Based on the research results, it can be concluded that there is a difference between the quality of the morphological staining results of spermatozoa using dragon skin extract and Papanicolaou, so several optimizations need to be carried out for maximum staining